NOVEL SMALL MOLECULAR COMPOUND FOR THE GENERATION MAINTENANCE PROLONGED UNDIFFERENTIATED GROWTH OF PLURIPOTENT STEM CELLS COMPOSITION COMPRISING THE SAME AND METHOD OF CULTURING PLURIPOTENT STEM CELL USING THE SAME

摘要

According to the present invention, the addition of a novel low molecular compound RSC-133 to de-differentiation induction culture process for producing pluripotent stem cells from human differentiated cells, to promote de-differentiation inducing efficiency It can dramatically reduce the time spent at the station, as well as induce differentiation. In particular, the de-differentiation inducing factor and cancer was found to be substituted for the risk factors c-Myc, it was confirmed that effective to promote all of the de-differentiation inducing efficiency in normal oxygen culture conditions and hypoxic culture conditions. In addition, RSC-133 inhibit the aging caused induction of de-differentiation induction process and shows the effect of promoting cell proliferation, slowing, weather resistance by inducing activation genetically improve the de-differentiation induction culture conditions and that the verified. The present invention is a patient of small amounts secured from a variety of sources - and improve the specific somatic cells derived from optimizing the production process of pluripotent stem cells applied clinically by contributing personally tailored stem cells, cell therapy development and drug development process significantly, practical It will contribute to accelerating the time. And In another aspect, the present invention, a novel low molecular compound RSC-133 was confirmed that the composition can be an effective culture medium for maintenance of undifferentiated pluripotent stem cells typical of human ES cells. RSC-133 is added to the medium composition is able to induce the undifferentiated proliferation of human embryonic stem cells effectively be expected to be useful in the development of embryonic stem cells in mass culture systems. ;