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SYSTEM AND METHOD FOR CULTURING SINGLE CELL IN FINE DROPLET

机译:培养细小液滴中单细胞的系统和方法

摘要

The present invention relates to a cell culture system and method, and more particularly, to a cell culture system and method for providing a single cell-based cell culture environment. According to the present invention, there are provided a first flow path formed so that a first liquid containing a large number of single cells flows; A second flow path connected to the first flow path such that the second liquid flows and the first liquid joins the second liquid in the middle; A gene transfer unit connected to an upstream end of the first flow path and transferring a specific gene to each of the single cells to induce transformation; And a cell culture unit connected to a downstream end of the second flow path, wherein the first liquid flows into the second flow path from the first flow path and interacts with the second liquid to include one single cell therein And the cell culture unit receives the plurality of micro droplets supplied from the second flow path to cultivate a single cell, and the microdroplets in which micro-algae that have been successfully cultured by electrophoresis or the like are present, A single cell culture system in a microdroplet, and a method for culturing a single cell in a microdroplet using the same.
机译:本发明涉及细胞培养系统和方法,更具体地说,涉及用于提供基于单细胞的细胞培养环境的细胞培养系统和方法。根据本发明,提供了第一流动路径,该第一流动路径被形成为使得包含大量单细胞的第一液体流动;第二流道连接到第一流道,使得第二液体流动并且第一液体在中间汇合第二液体;基因转移单元,其连接至第一流路的上游端,并且将特定基因转移至每个单细胞以诱导转化;和细胞培养单元,其连接至第二流动路径的下游端,其中第一液体从第一流动路径流入第二流动路径并与第二液体相互作用以在其中包括单个细胞,并且细胞培养单元接收从第二流路供给的多个微滴用于培养单细胞,并且存在其中已经通过电泳等成功培养的微藻类的微滴,微滴中的单细胞培养系统和方法使用相同的微滴培养单个细胞。

著录项

  • 公开/公告号KR101605947B1

    专利类型

  • 公开/公告日2016-03-23

    原文格式PDF

  • 申请/专利权人 한국과학기술원;

    申请/专利号KR20140025784

  • 发明设计人 서태석;배선웅;

    申请日2014-03-05

  • 分类号C12M3;

  • 国家 KR

  • 入库时间 2022-08-21 14:12:46

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