B12 Method for production of coenzyme B12 by recombinant Escherichia coli

摘要

The present invention relates to a method for producing coenzyme B ₁₂ using recombinant E. coli, wherein the recombinant E. coli developed by the present invention is capable of producing coenzyme B ₁₂ itself and is capable of producing coenzyme B ₁₂ Can be obtained, and the production of coenzyme B ₁₂ can be improved by optimizing the culture conditions of the recombinant E. coli. In addition, since the recombinant E. coli according to the present invention can produce coenzyme B ₁₂ by itself, it can be produced by using an enzyme such as glycerol dihydrate (DhaB), which requires coenzyme B _12, which is expensive as coenzyme and 3-hydroxypropionic acid, It can be used as an economical and valuable host strain in the production of commercially important chemicals such as 3-propanediol.