L- A mutant of Escherichia coli sugar isomerase improved in its activity and production of L-gulose using the said enzyme

摘要

the invention of quantum mechanics and molecular dynamics techniques and technologies used in part mutants of E. coli O157: H7 ( Escherichia coli O157: H7 ) to increase the activity of the topoisomerase-derived sugar. More specifically, the quantum mechanics and looking at residues affecting the enzyme stability through screening based on molecular dynamics, topoisomerase The enzyme activity improved by mutation of the found sugar moiety, the nucleic acid molecule encoding it, the nucleic acid molecule comprising a vector, a transformant containing the vector, using the mutants and improved topoisomerase per topoisomerase of the sugar to a method for producing L- gulose. ;