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LYTIC PROTEINS OF VERO CELL PREPARATION METHOD THEREOF AND DETECTION KIT FOR VERO HCP COMPRISING THE SAME
LYTIC PROTEINS OF VERO CELL PREPARATION METHOD THEREOF AND DETECTION KIT FOR VERO HCP COMPRISING THE SAME
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机译:包含其的Vero HCP的Vero细胞制备方法及其检测试剂盒的溶蛋白
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摘要
The present invention relates to a method for culturing and harvesting a soluble protein of Vero cells, and Vero cells; Destroying and dissolving Vero cells; Purifying the soluble protein of Vero cells; And concentrating to obtain a soluble protein of Vero cells. The soluble proteins of Vero cells prepared according to the method of the present invention have a wide range of applications. They have high sensitivity and good repeatability and can be used for quality control and analysis in the production of vaccines using Vero cells. The present invention also provides Vero cell HCPs assay kits with strong specificity, high sensitivity, and excellent repeatability. This can be used for quality control and analysis in the production of non-secreted virus vaccines against viruses such as hepatitis A virus, as well as other vaccines produced using Vero cells.
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