4 Tetraploid induction Method of watermelon variety

摘要

TECHNICAL FIELD The present invention relates to an efficient tetraploid organic method by chemical treatment of seedlings secured by in vitro germination of watermelon seeds, a) germinating and sterilizing the sterilized watermelon seeds in MS medium; b) treating the medicament of colchicine and 2,6-dinitroaniline at the seedling growth point of step a); c) cutting the cotyledon and the growth point of the medicament-treated seedlings of step b) to obtain a slice, and dipping the resulting slice on an MS medium to induce shoots; d) elongating the shoot of step c) in an MS medium; e) rooting induction of the elongated shoot of step d) in MS medium; f) purifying the rooted watermelon plant of step e) into a soil environment; g) transplanting the purified watermelon plants of step f) in pots and growing them; .