TETRAPLOID INDUCTION METHOD OF WATERMELON VARIETY

摘要

The present invention relates to a method for efficiently cultivating tetraploid watermelon varieties by chemical treatment in a seedling obtained by in vitro germination of watermelon seeds. The method according to the present invention comprises the following steps of: (a) germinating sterilized watermelon seeds in an MS culture medium and forming a seedling; (b) treating growing points of the seedling in the step (a) with chemicals of colchicine and 2,6-dinitroaniline; (c) obtaining fragments by cutting cotyledons and growing points of the seedling treated with chemicals in the step (b), and planting the obtained fragments in the MS culture medium to induce shoots; (d) growing the shoots in the step (c) in the MS culture medium; (e) inducing the rooting of the grown shoots in the step (d) in the MS culture medium; (f) purifying the rooted watermelon plant bodies in the step (e) to the soil environment; and (g) transplanting the purified watermelon plant bodies in the step (f) into a flowerpot and growing the same.