GENETIC MARKER FOR DISCRIMINATING KOI HERPESVIRUS GRAY SPH, AND METHOD FOR DISCRIMINATING CAUSATIVE VIRUS BY USING SAME

摘要

The present invention relates to a genetic marker for discriminating koi herpesvirus gray SPH (HSV gray SPH), and to a method for discriminating a causative virus by using the same. More specifically, the method comprises: selecting and amplifying a DNA base sequence for coding a particular gene of HSV gray SPH; hybridizing peptide nucleic acid (PNA) which specifically recognizes an amplified product; obtaining melting curves by temperature by adjusting a temperature of a hybridized product; and discriminating HSV gray SPH or discriminating infection of fish by the virus from a melting temperature by analyzing the obtained melting curves.;COPYRIGHT KIPO 2016