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Probe set for diagnosing or detecting Southern bean mosaic virus and uses thereof

机译:诊断或检测南方豆花叶病毒的探针组及其用途

摘要

The invention provides oligonucleotides for SBMV (Southern bean mosaic virus) diagnostics or detection comprising one or more oligonucleotides selected from the group consisting of oligonucleotides of SEQ ID NOS: 3024 to 3028 and SEQ ID NOS: 3057 to 3060 and complementary oligonucleotides thereof A probe set, a gene chip for SBMV diagnosis or detection comprising the oligonucleotide probe set, separating RNA from a sample presumed to be infected with SBMV, synthesizing cDNA using the separated RNA as a template, A method for diagnosing or detecting SBMV comprising contacting a cDNA with a set of oligonucleotide probes to hybridize the cDNA and a probe set and detecting the degree of hybridization between the probe and the cDNA and a method for diagnosing or detecting SBMV comprising the oligonucleotide probe set and hybridizationIt relates to SBMV or detection kit comprising the solution.
机译:本发明提供了用于SBMV(南豆花叶病毒)诊断或检测的寡核苷酸,其包含一种或多种选自SEQ ID NOS:3024至3028和SEQ ID NOS:3057至3060的寡核苷酸及其互补的寡核苷酸的探针组一种用于SBMV诊断或检测的基因芯片,其包括寡核苷酸探针组,从假定感染SBMV的样品中分离RNA,使用分离的RNA作为模板合成cDNA。一种用于诊断或检测SBMV的方法,包括将cDNA与用于与cDNA和探针组杂交的寡核苷酸探针组以及检测探针和cDNA之间的杂交程度的方法以及用于诊断或检测包含该寡核苷酸探针组并杂交的SBMV的方法本发明涉及SBMV或包含该溶液的检测试剂盒。

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