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Probe set for diagnosing or detecting Squash mosaic virus and uses thereof

机译:诊断或检测南瓜花叶病毒的探针组及其用途

摘要

The present invention provides an oligonucleotide probe set for diagnosis or detection of SqMV (squash mosaic virus) comprising at least one oligonucleotide selected from the group consisting of oligonucleotides of SEQ ID NOS: 894 to 909 and complementary oligonucleotides thereof, , Isolating RNA from a sample suspected of being infected with SqMV, synthesizing cDNA using the separated RNA as a template, synthesizing the synthesized cDNA with the oligonucleotide probe set And detecting the degree of hybridization between the probe and the cDNA; and a method for diagnosing or detecting SqMV, comprising the steps of: (a) detecting a hybridization state between the probe and the cDNA;Chulyong relates to the kit.
机译:本发明提供了一种用于诊断或检测SqMV(南瓜花叶病毒)的寡核苷酸探针组,其包含至少一种选自SEQ ID NOS:894至909的寡核苷酸及其互补寡核苷酸的寡核苷酸,从样品中分离RNA。怀疑感染了SqMV,以分离的RNA为模板合成cDNA,用寡核苷酸探针组合成合成的cDNA,并检测探针与cDNA之间的杂交程度;一种用于诊断或检测SqMV的方法,包括以下步骤:(a)检测所述探针与所述cDNA之间的杂交状态。

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