Protease Sensors and Protease Activity Assay Method Using the Protease Sensors

摘要

The present invention relates to a sensor used to measure protease activity, and a measurement method of protease activity using the same. According to the present invention, the measurement method of protease activity using recombinant pro-caspase solves various problems which occur in an existing measurement method of protease activity, like high background noise signals, interference (disturbance) between biological samples, the use of unrefined protein, and difficulties in using an experiment apparatus. The measurement method of protease activity is accurate and sensitive and secures high user convenience. Accordingly, the measurement method of protease activity can be used as a standard protocol for measuring protease activity, and further can be developed into a disease diagnosis method. Recombinant pro-caspase-3 includes: a linker including an amino acid sequence which is cleaved by matrix metalloproteinases (MMP) 2. The linker is at least one selected from the group consisting of GPLGVR, GIETGPLGVRS, GIETGSGPLGVRGS, GIETGGSGPLGVRGGS, and GIETGGGSGPLGVRGGGS.