The production of enzymes in the conditions of deficiency of simple sugars OF lignocellulosic biomass

摘要

1. A method of producing a host cell with at least one enzyme to hydrolyze first pretreated lignocellulosic biomass, wherein said method comprises stadiyukultivirovaniya host cell capable of producing at least one enzyme for the culture time, and, culturing the host cells carried out in a medium for culturing soderzhascheykletku host, which is a Penicillium cell decumbensvtoruyu pretreated lignocellulosic bi omassu comprising complex sugar, said culturing is performed under conditions of deficiency of simple sugars with the optional addition of simple sugars or sugar in an amount of from 0 to 10% by weight of said culture medium, based on dry substance during part of said culturing time of at least 50% of this culture time; iukazannaya culture medium is substantially devoid of added vitamins, minerals, salts, minerals, lactose, and isopropyl β-D-1-thiogalactopyranoside; imassovoe solids content of said second pretreated lignocellulosic biomass in said culture medium exceeds 4.2. The method of claim. 1, characterized in that the weight content of the dry substance of said second pretreated lignocellulosic biomass in said culture medium than a value selected from the group consisting of 6%, 8%, 10%, 15% .3. A method according to any one of claims. 1 and 2, characterized in that said portion of the culture time in conditions of deficiency n