ANALYSIS ADROMEDULLINA and method for determining MATURE ADROMEDULLINA

摘要

1. A method for in vitro monitoring of therapy in patients who are suspected of sepsis, wherein the concentration of mature ADM 1-52 and / or mature ADM 1-52-Gly in a sample of biological fluid from said patient with sepsis is determined using the assay comprising two binders that bind to two different areas within a region of the mature adromedullina and / or adromedullina-Gly, which is a 21-52 amino acid-amide (SEQ ID No. 1) or amino acid 21-52-Gly (SEQ ID No. 2), wherein each of said regions comprises at least 4 or 5 -amine kislot.2. In vitro method for monitoring therapy in patients with sepsis according to Claim. 1, wherein one of said binder binds to the region containing the following sequence of mature ADM and / or mature ADM l-52-Gly (SEQ ID No. 4), and wherein the data of said second coupling is associated with the region contained in the sequence of mature ADM and / or mature ADM 1-52-Gly (SEQ ID No. 5) .3. A method in vitro for monitoring therapy in patients with sepsis of Claim. 1 or 2, wherein the sensitivity of this assay is sufficient to quantify the ADM in healthy subjects and is 10 pg / ml, preferably 40 pg / ml, and more preferably 70 pg / ml.4. In vitro method for monitoring therapy in patients with sepsis of claim. 1 or 2, wherein said binder exhibits a binding affinity to the mature ADM and / or mature ADM l-52-Gly, at least 10M.5. In vitro method for monitoring therapy in patients with sepsis of claim. 1 or 2, wherein said binder is selected from the group consisting of anti-adromedullinovoe antibody or a fragment of an anti-ADM antibody Binding