Microorganisms of the genus ESCHERICHIA, has an enhanced ability to produce L-tryptophan, and methods for producing L-tryptophan with its use

摘要

1. A recombinant microorganism of the genus Escherichia, which has enhanced ability to produce L-tryptophan, wherein said recombinant microorganism is modified by deletion of part or all of the leader peptide having the nucleotide sequence of SEQ ID NO: 2, in the expression regulatory region having a nucleotide sequence SEQ ID NO: 1, at operone.2 endogenous tryptophan. The recombinant microorganism of claim 1, wherein said recombinant mikroorgan. Edited was further modified by deletion of part or all of the endogenous attenuator having the nucleotide sequence of SEQ ID NO: 3, in an expression regulatory region having the nucleotide sequence of SEQ ID NO: 1.3. Recombinant microorganism according to Claim. 1 wherein said recombinant microorganism has been further modified to enhance the activities of the proteins encoded by the tryptophan operonom.4. . The recombinant microorganism of claim 1, wherein said recombinant microorganism has been further modified to increase activities of one or more proteins having the amino acid sequence of SEQ ID NO: 37, 38, 39 and 40, respectively, which are encoded by the tryptophan biosynthetic gene cluster trpDCBA.5. The recombinant microorganism of claim. 3 or 4, wherein the activity of said proteins povyshayut method of increasing the copy number of chromosomal or intracellular genes encoding proteins by substitution the regulatory region of the gene on the chromosome of a strong promoter or by regulatory expression domain modification to obtain regulatory expression domain with enhanced activity. 6. Recombinant microorganism according to Claim. 1 wherein said recombinant microorganism pre