The method of modeling of peritonitis in the experiment

摘要

The method of modeling experiments in peritonitis in rats by administration into the abdominal cavity of a slurry of microbial cultures in a volume of 20.0 ml, 1.0 kg of animal body weight, characterized in that in the peritoneal cavity of an experimental animal broth mixture slurry is introduced at the rate of 6 billion mic. per 100 g body weight of animal organisms Escherichia coli and Staphylococcus aureus, isolated from patients with acute appendicitis or acute cholecystitis or acute pancreatitis, peritonitis complicated, in a ratio of: Escherichia coli - 60% concentration of 3,6 × 10mikrobnyh bodies per 100 g of animal weight, Staphylococcus aureus - 40% at a concentration of 2,4 × 10mikrobnyh bodies per 100 g of animal weight, followed by administration of the culture broth - meat-peptone broth in a final volume to 20.0 ml per 1.0 kg of animal body weight.