STRAIN O №2108/ZABAIKALSKY/2010 OF FOOT-AND-MOUTH DISEASE VIRUS Aphtae epizooticae OF TYPE O FOR MANUFACTURING BIOLOGICAL PREPARATIONS FOR DIAGNOSTICS OF FOOT-AND-MOUTH DISEASE VIRUS OF TYPE O

摘要

FIELD: veterinary medicine.;SUBSTANCE: picornaviridae, of genus Aphtovirus, deposited in the collection of FSBI "ARRIAH" under the registration number of foot-and-mouth disease virus of type O No. 2108/Zabaikalsky/2010. The presented strain is reproduced in primary trypsinised monolayer porcine kidney (PK) cell culture, passaged cultures of kidney cells of Siberian mountain goat (SMGK-30) and porcine kidney (IB-RS-2). During the 18-24 hours of incubation the virus yield in the said cell cultures reaches values of 6.25-6.75 lg 50% TCD₅₀/cm³. At high multiplicity of infection (1-10 TCD/cell) causes TCID after 5 hours, while maintaining the initial characteristics when passaging in cell cultures for 10 passages.;EFFECT: strain may be used to control the antigenic and immunogenic activity of foot-and-mouth vaccines and for manufacture of biological products for diagnostics of foot-and-mouth disease of type O.;5 cl, 1 dwg, 7 tbl, 4 ex