VIRUS-LIKE PARTICLES CONTAINING TARGET PROTEINS FUSED WITH COAT PROTEINS OF PLANT VIRUSES

摘要

FIELD: medicine, pharmaceutics.SUBSTANCE: presented group of inventions concerns a virus-like particle and methods for using and producing the same. The presented virus-like particle substantially free from viral nucleic acid is formed of a fused protein containing a coat protein of a plant virus, and a target protein. The target protein is produced of a cell surface polypeptide of an intracellular pathogen, whereas the coat protein of the plant virus is specified in a group of Alfalta mosaic virus, Aleppo grass mosaic virus and potato virus Y. Immunogenic formulations containing these virus-like particles may be introduced into patient's body to stimulate protective immune responses. The method for producing the virus-like particles involves a process of fused protein expression in a host cell in the environment adequate to enable assembling the virus-like particle from the above fused protein.EFFECT: characterised inventions enable reproducing a desired antigen determinant or a foreign peptide on the surface of the virus-like particle free from nucleic acid, for the purpose of immunisation.24 cl, 13 dwg, 3 tbl, 9 ex