Confocal 4 - pi - microscope and method for confocal 4 - pi - microscopy

摘要

A method for confocal 4 - pi - microscopy, characterized by the following steps:• Coherent illuminating a sample of two sides in each case by means of a lens with the illumination light, the at least one illumination waves length, by interference of the illumination light in the sample a standing illuminating wave with a lighting - primary maximum and with lighting - secondary maxima is generated;• Detecting the detection light emanating from the sample, the at least one detection wavelength by means of the two lenses, wherein the detection light is brought to interference and, as a result, a detection in the sample pattern with a detection - primary maximum and with detection - secondary maxima is produced in such a way that the lighting - secondary maxima and the detection - secondary maxima at different locations are located; and• Marking of the sample with at least one fluorescent dye, which is selected in such a way that the ratio of illumination waves length to detection wavelength in the range of 0,5 to 0,9 or in the range from 0,6 to 0,8 or in the case of 0,75 is;• Provision of a detection pinhole, the diameter of less than 1 airy disc or of 0,7 to 0,8 airy disks or of 0,7 airy disks is.