Method and grid fluorescence light microscope for three-dimensionally high-resolution imaging a with fluorophores marked structure of a sample

摘要

For the three-dimensionally high-resolution imaging a with switchable fluorophores marked structure in a sample (3) are, according to the arrangement of the sample (3) in front of a lens (4) repeats the steps are carried out: straightening of fluorescence order to permit light (34) along the focal plane (5) of the objective lens (4) on the sample (3) along the focal plane (5) a light sheet (19) from the fluorescence light (34) to make it possible to design, of partial regions of the light sheet (19) to prevent with fluorescence light (35), which is a zero point within the light sheet (19), wherein the fluorescence light (35) to prevent a component (13), which the zero point in the direction orthogonal to the focal plane (5) is limited, and a further component (14) through the objective lens (4) on the sample (3) is directed, and the zero point in at least one direction of the focal plane (5) of the objective lens (4) is limited; measuring from the zero point of the sample (3) and emitted with the objective lens (4) the detected fluorescence light (8); associating of the measured fluorescent light (8) to the location of the zero point in the sample (3) in the direction orthogonal to the focal plane (5) and in the at least one direction of the focal plane (5) of the objective lens (4); and displacement of the zero position with respect to the sample (3).