METHOD FOR ENZYMATIC HYDROLYSIS OF HIGH MOLECULAR-WEIGHT NATURAL POLYMER

摘要

Use: present invention relates to the fields of biotechnology and medicine, more particularly to processes of enzymatic hydrolysis of high-molecular natural polymers, and it may be useful in medicine, agriculture, pharmaceutics, cosmetics. Objective: providing a novel process of enzymatic hydrolysis of a high-molecular natural polymer by means of autofermentation, while providing for optimal conditions for a progress of enzyme-substrate reaction. Summary of invention: a process of enzymatic hydrolysis of a high-molecular natural polymer, comprising a preparatory stage with an addition of distilled water and homogenate heating, performing hydrolysis while stirring continuously and assessing a quality of a hydrolyzate thus produced, wherein according to the invention honey is used as said high-molecular natural polymer which is exposed to an addition of 80% of purified water as compared to a total volume of water to be added, a temperature is raised up to 35 - 40°C, and a pH of the homogenate is adjusted up to 6.0, then the temperature is further raised under intensive stirring and pH = 6.0 to make 45°C, a pH value is adjusted every 10 minutes for a period of 50 - 60 minutes in order to achieve a stabilized pH value within a range of 4.5 - 5.0, then a resultant hydrolyzate is cooled down to room temperature, exposed to stirring, subjected to addition of ethanol used as a stabilizer in an amount of 2% of a total volume of the resultant hydrolyzate, the remaining water is subsequently added, the resultant hydrolyzate is stirred again, settled for 60 minutes to produce a defermented honey concentrate which is subsequently filtered, poured into containers and autoclaved at 110°C.