HIGHLY FUNCTIONAL ENZYME HAVING MODIFIED SUBSTRATE SPECIFICITY OF HUMAN BETA-HEXOSAMINIDASE B AND EXHIBITING PROTEASE RESISTANCE

摘要

Provided is a modified ²-subunit of human ²-hexosaminidase which has the activity derived from the ±-subunit of wild-type human ²-hexosaminidase and has the resistance to protease. A protein comprising an amino acid sequence having substitutions of the 312th to the 318th amino acids with glycine, serine, glutamic acid, proline, serine, glycine and threonine in order, respectively, in an amino acid sequence of a ²-subunit of wild-type human ²-hexosaminidase.