PCR-RFLP-BASED METHOD FOR IDENTIFYING, AND DETERMINING THE PURITY OF PISCIRICKETTSIA SALMONIS

摘要

The present invention refers to a rapid, sensitive, specific, reproducible and low cost method for identifying and determining purity of Piscirickettsia salmonis bacterium. This method is a method based on PCR-RFLP (Complete) to identify Piscirickettsia salmonis and to establish as well if a sample of Piscirickettsia salmonis (for example, a bacterial culture) is pure or contaminated with other bacteria that could cohabit with Piscirickettsia salmonis. In this way, this method could be set up in laboratories all over the world as a standard technology to monitor bacterial cultures and in technologies for the epidemiologic control of Piscirickettsia salmonis. The present invention protects as well a kit for identifying and determining purity of Piscirickettsia salmonis.