METHOD FOR SELECTING (POLY) PEPTIDE/PROTEIN TAG CAPABLE OF COVALENTLY BONDING TO ANY TARGET SUBSTANCE AND (POLY) PEPTIDE/PROTEIN TAG SELECTED AND OBTAINED

摘要

PROBLEM TO BE SOLVED: To provide a method for obtaining at high speed a (poly) peptide/protein tag for protein-labeling, which is covalently bonded to a target substance.SOLUTION: The present invention provides a method for selecting a peptide or a protein, which is capable of covalently bonding to any target substance. The method comprises the steps of: (a) preparing an RNA library by transcribing a DNA library; (b) preparing a library of RNA-linker-nucleic acid derivatives; (c) performing a s peptide or protein synthesis in a cell-free translation system to produce an mRNA display peptide/protein library; (d) reverse-transcribing an mRNA part to DNA to prepare a cDNA display peptide/protein library; (e) contacting the cDNA display peptide/protein library with an immobilized target substance; and (f) selecting a conjugate in which a peptide portion or a protein portion of the cDNA display peptide/protein library is covalently bonded to the target substance.SELECTED DRAWING: Figure 1