METHOD FOR PRODUCING TRANSGENIC PLANT FOR PREPARING GENETICALLY MODIFIED PLANT AND GENETICALLY MODIFIED TRANSFORMED PLANT PRODUCED THEREBY

摘要

PROBLEM TO BE SOLVED: To provide a method for producing a transformed plant, the method being capable of effectively introducing a gene into a rubber-producing plant in a short period of time while retaining useful traits of the transgenic plant, and the resulting plant being capable of effectively redifferentiating, thereby producing a genetically modified plant.SOLUTION: According to the present invention, a method for producing a transgenic plant for producing a genetically modified plant comprises: a culture step in which a tissue piece derived from a rubber-producing plant is cultured to obtain a cultured tissue piece; a transformation step in which the cultured tissue piece obtained in the culture step is transformed using a gene construct; and a regeneration step in which a plant is regenerated from the tissue piece transformed in the transformation step. The transformation step includes an infection step in which the cultured tissue piece is cultured together with an Agrobacterium strain transformed with a gene construct, and a bacteria-removing step in which the cultured tissue piece obtained in the infection step is cultured in a bacteria-removing medium containing a Carbapenem antibiotic.SELECTED DRAWING: None