TRANSGENIC PLANT PRODUCTION METHOD FOR PRODUCING GENETICALLY MODIFIED PLANT AND GENETICALLY MODIFIED TRANSGENIC PLANT PRODUCED BY SAID PRODUCTION METHOD

摘要

Provided is a transgenic plant production method for producing a genetically modified rubber-producing plant that is capable of efficient short-term gene transfer, of introducing a target gene while retaining the useful traits of a plant undergoing gene transfer, and of efficient redifferentiation. The method includes: a culture step of culturing a tissue fragment from a rubber-producing plant to obtain a cultured tissue fragment; a transformation step of transforming, with a gene construct, the cultured tissue fragment obtained in the culture step; and a regeneration step of regenerating a plant from the tissue fragment transformed in the transformation step, wherein the transformation step includes: an infection step of culturing the cultured tissue fragment in the presence of an Agrobacterium transformed with a gene construct; and a decontamination step of culturing the cultured tissue fragment obtained in the infection step in a decontamination medium containing a carbapenem antibiotic to decontaminate the Agrobacterium.