IMMORTALIZED NEURONAL CELL LINE DERIVED FROM HYPOTHALAMIC REPRODUCTIVE CENTER IN LIVESTOCK

摘要

PROBLEM TO BE SOLVED: To provide methods of establishing an immortalized cell line from KNDy neuron or GnRH neuron which are derived from the hypothalamus of a ruminant livestock, such as goats, cattle, and the like, which controls reproductive and reproductive activities of livestock.;SOLUTION: Disclosed is an immortalized neuronal cell line derived from a hypothalamus, preferably derived from a KNDy neuron or a GnRH neuron, in which the immortalization of a neuron is achieved by expressing SV40T-antigen and further in which the cell clone is selected using the expression of each neuronal marker as an indicator. A screening method for a drug having the effect on reproduction of ruminant livestock comprises contacting a candidate compound with an immortalized neuronal cell line.;SELECTED DRAWING: Figure 1;COPYRIGHT: (C)2017,JPO&INPIT