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Novel bacteriophage exhibiting lytic properties against Salmonella and compositions containing the same

机译:表现出针对沙门氏菌的裂解特性的新型噬菌体及其组合物

摘要

PROBLEM TO BE SOLVED: To provide a bacteriophage showing bacteriolytic activity to Salmonella, and a bacteriophage composition.SOLUTION: A bacteriophage is characterized in that: (1-a) The bacteriophage is derived from poultry; (1-b) The bacteriophage belongs to Myoviridae; (1-c) The bacteriophage has bacteriolytic activity to at least S. Entetitidis, S. Typhimurium, S. Gallinarum, S. Pullorum, S. Infentis and S. Thompson; (1-d) When a solution including the bacteriophage incubated at 95°C for 5 min in the presence of SDS is subjected to electrophoresis with a gel having cut-off molecular weight of 10,000 to 150,000, the band of protein are detected at 60.1±1.0 kDa, 54.7±1.0 kDa, 42.4±1.0 kDa, 15.8±1.0 kDa and 14.8±1.0 kDa; and (1-e) The size of genome DNA is 84.5±1.0 kbp. A composition contains the bacteriophage.
机译:解决的问题:提供一种对沙门氏菌具有溶菌活性的噬菌体和一种噬菌体组合物。解决方案:噬菌体的特征在于:(1-a)噬菌体来源于家禽; (1-b)噬菌体属于肌病毒科; (1-c)所述噬菌体至少对肠炎沙门氏菌,鼠伤寒沙门氏菌,鸡沙门氏菌,普鲁托鲁姆菌,英菲尼迪斯菌和汤普森菌具有溶菌活性; (1-d)当将含有在SDS存在下在95℃下孵育5分钟的噬菌体的溶液用截留分子量为10,000至150,000的凝胶进行电泳时,在60.1处检测到蛋白质条带±1.0 kDa,54.7±1.0 kDa,42.4±1.0 kDa,15.8±1.0 kDa和14.8±1.0 kDa; (1-e)基因组DNA的大小为84.5±1.0kbp。组合物包含噬菌体。

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