Methods for isolating, culturing, and genetically engineering immune cell populations for adoptive therapy

摘要

The disclosure relates in some aspects to methods, cells, and compositions for preparing cells and compositions for genetic manipulation and cell therapy. In some aspects, streamlined cell preparation methods are provided, eg, for isolation, processing, incubation, and genetic manipulation of cells and cell populations. Also provided are cells and compositions produced by the methods, and methods of their use. The cells can include immune cells such as T cells and generally include multiple isolated T cell populations or T cell types. In some aspects, the method may prepare multiple different cell populations for adoptive therapy using fewer steps and / or resources and / or reduced handling procedures compared to other methods. Is possible.