Detection of target nucleic acid sequences using PTO cleavage and extension-dependent signaling oligonucleotide cleavage {DetectionofTargetNucleicAcidSequencebyPTOCleavageandExtension-DependentSignalingOligonucleotideCleavage}

摘要

The present invention relates to the detection of a target nucleic acid sequence by a PTO Cleavage and Extension-Dependent Signaling Oligonucleotide Cleavage assay (PCE-SC assay). The present invention is carried out in such a manner that the extended strand is produced on the CTO having arbitrary sequences as templates depending on the presence of target nucleic acid sequences and in turn the SO as probes is hybridized with the extended strand to give signal. The present invention employs a series of reactions including PTO hybridization and cleavage, CTO hybridization and extension, and SO hybridization and cleavage, which is responsible for the highly enhanced specificity of the present invention.