Serum-free medium for culturing the digestive system cancer stem cells, and methods of proliferation digestive system cancer stem cells using the same

摘要

PROBLEM TO BE SOLVED: To provide a serum-free medium for culturing digestive system cancer stem cells, a method for culturing digestive system cancer stem cells, particularly a method for stably proliferation culturing for a long period of time.SOLUTION: Digestive system cancer stem cells are proliferated for over three months by suspension culturing cells isolated from a digestive system cancer cell specimen in a serum-free medium added with transferrin, insulin, putrescine, progesterone, an epidermal growth factor (EGF), a basic fibroblast growth factor (Basic FGF) and a leukemia inhibitory factor (LIF) in addition to neural survival factor-1, to form a cell aggregate (Sphere) of the digestive system cancer stem cells, isolating the sphere, adhesion culturing the same in the serum-free culture medium by using an incubator coated with cell adhering molecules, and continuing the culture by gradually substituting a part of the serum-free medium with a fresh serum-free medium.