Method for imparting acid and salt tolerance and production of useful substances using acid and salt tolerant yeast

摘要

Strong acid (low pH) by adding acid and salt tolerance by introducing genes and proteins that are resistant to acids and salts from acid- and salt-resistant yeast into yeast with excellent fermentation production or improving their expression level Providing a method for producing useful substances such as ethanol using yeasts that exhibit various growth inhibition resistance and fermentation inhibition resistance in a high salt environment. Genetically modified yeast that constantly expresses LGS1 gene from Issatchenkia orientalis or LGS1 homologous gene belonging to GH72 family from other yeasts (GAS1, PHR1, and PHR2 genes) and low pH and high salt concentration using it A method for obtaining resistance under an environment and a method for producing useful substances such as ethanol with high efficiency from glucose or a saccharified solution containing glucose.