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Cell culture substrate, cell culture method using the same, and method for inducing differentiation of pluripotent stem cells

机译:细胞培养基质,使用其的细胞培养方法以及诱导多能干细胞分化的方法

摘要

PROBLEM TO BE SOLVED: To provide a method a cell culture substrate capable of culturing multipotent stem cells without using feeder cells, differentiating the multipotent stem cells to specific cells at high purity, and also being used for selecting cells after differentiation, and to provide a method for culturing cells using the same, and a method for inducing differentiation of the multipotent stem cells.SOLUTION: The cell culture substrate includes fixing or coating a protein belonging to cadherin family or a fusion protein containing all or a part of region of the protein belonging to cadherin family, and a polymer having a glycosylated chain on a surface thereof.
机译:解决的问题:提供一种细胞培养基质的方法,该基质能够在不使用饲养细胞的情况下培养多能干细胞,将多能干细胞以高纯度分化为特定细胞,并且还用于在分化后选择细胞,并提供解决方法:细胞培养底物包括固定或包被属于钙粘蛋白家族的蛋白或包含该蛋白全部或部分区域的融合蛋白的方法属于钙粘着蛋白家族,并且在其表面上具有糖基化的链的聚合物。

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