Compositions and methods for identifying replica sequencing reads

摘要

The present invention provides methods, compositions and kits for detecting replica sequencing reads. In some embodiments, the replica sequencing read is removed. In one aspect, a method is provided for detecting a replica sequencing read from a population of sample sequencing leads, the method comprising: a) adapting an adapter to each of a plurality of nucleic acid fragments from one or more samples. Ligating to the 5 ′ end of a nucleic acid fragment, wherein the adapter comprises: (i) an indexing primer binding site; (ii) an indexing site; (iii) an identifier site; and (iv) a target sequence Including a primer binding site; b) amplifying the adapter-nucleic acid fragment ligation product; c) generating a population of sequencing reads from the amplified adapter-nucleic acid fragment ligation product, and the like. Include.