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SYSTEM FOR WIDE FIELD-OF-VIEW, HIGHLY OBLIQUE ILLUMINATION MICROSCOPY FOR SCATTER-BASED DISCRIMINATION OF CELLS
SYSTEM FOR WIDE FIELD-OF-VIEW, HIGHLY OBLIQUE ILLUMINATION MICROSCOPY FOR SCATTER-BASED DISCRIMINATION OF CELLS
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机译:基于视野的高倾斜照明显微系统,用于基于散射细胞的细胞识别
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摘要
System for scatter-based differentiation of cells. The system includes a planar substrate having cells disposed thereon for imaging and a microscope employing relatively low magnification detection optics providing a wide field of view focused on the cells on the substrate and defining a microscope optical axis. An illuminator delivers light to the ceils on the substrate focused to a spot larger than the field of view of the microscope, the illuminator having an illumination optical axis highly oblique to the microscope optical axis wherein scattered light is collected by the microscope. A defection system receives the scattered light from the microscope to provide a measure of signal intensity and a measure of size for each cell for discrimination of cell type.
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