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COMPETITIVE COMPOSITIONS OF NUCLEIC ACID MOLECULES FOR ENRICHMENT OF RARE-ALLELE-BEARING SPECIES
COMPETITIVE COMPOSITIONS OF NUCLEIC ACID MOLECULES FOR ENRICHMENT OF RARE-ALLELE-BEARING SPECIES
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机译:富含稀有等位基因的核酸分子的竞争性组成
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摘要
The present disclosure describes the thermodynamic design and concentrations necessary to design probe compositions with desired optimal specificity that enable enrichment, detection, quantitation, purification, imaging, and amplification of rare-allele-bearing species of nucleic acids (prevalence 1%) in a large stoichiometric excess of a dominant-allele-bearing species (wildtype). Being an enzyme-free and homogeneous nucleic acid enrichment composition, this technology is broadly compatible with nearly all nucleic acid-based biotechnology, including plate reader and fluorimeter readout of nucleic acids, microarrays, PCR and other enzymatic amplification reactions, fluorescence barcoding, nanoparticle-based purification and quantitation, and in situ hybridization imaging technologies.
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