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Glycosylated modified flavin adenine dinucleotide-dependent glucose dehydrogenases, compositions thereof as well as methods of making and using the same
Glycosylated modified flavin adenine dinucleotide-dependent glucose dehydrogenases, compositions thereof as well as methods of making and using the same
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机译:糖基化修饰的黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶,其组合物及其制备和使用方法
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摘要
Compositions, devices, kits and methods are disclosed for assaying glucose with a glycosylated, modified flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH), variant thereof or an active fragment thereof, where at least one asparagine residue at positions N2, N168 and N346 of mature, wild-type A. oryzae FAD-GDH according to SEQ ID NO:2 is substituted by one or more amino acids not suitable for glycosylation, thereby eliminating or inactivating, respectively, a potential glycosylation site at this position.
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机译:公开了用于用糖基化的,修饰的黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶(FAD-GDH),其变体或其活性片段测定葡萄糖的组合物,装置,试剂盒和方法,其中至少一个天冬酰胺残基在位置N2,N168和成熟的野生型A的N346。根据SEQ ID NO:2的稻米FAD-GDH被一个或多个不适合糖基化的氨基酸取代,从而分别消除或灭活了该位置上的潜在糖基化位点。
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