Enhanced sensitivity of detection in electrospray ionization mass spectrometry using a post-column modifier and a microfluidic device

摘要

A microfluidic liquid chromatography-electrospray ionization (LC-ESI) device is provided for enhancing the sensitivity of mass spectrometric detection of an analyte in a sample. The device is designed to drive effective intermixing of an analytical flow stream exiting a chromatographic stationary phase and a post-column modifier reagent. The mixed flow stream thus obtained is used for generating an electrospray containing analyte ions. Also provided are methods for enhanced sensitivity of detection of an analyte in a sample.