首页>
外国专利>
DNA fragment detection method, DNA fragment detection kit and the use thereof
DNA fragment detection method, DNA fragment detection kit and the use thereof
展开▼
机译:DNA片段检测方法,DNA片段检测试剂盒及其用途
展开▼
页面导航
摘要
著录项
相似文献
摘要
The disclosure claims a cleaved Deoxyribonucleic acid (DNA) detection method, a DNA fragment detection kit and use thereof. Wherein, the method includes the steps of: designing primers according to a test site or a test region of the DNA fragment; cyclizing the DNA fragment to obtain acyclized DNA; implementing Polymerase Chain Reaction (PCR) amplification for the cyclized DNA by using the primers; and detecting the PCR amplification product. In the disclosure, by cyclizing the DNA fragment, the amplification can be implemented even if only one PCR primer can match with a template, thus, the adaption range and effective template amount of the primer amplification can be greatly increased, and the detection sensitivity of the DNA fragment can be greatly improved.
展开▼