methods and kit for detecting a target nucleic acid sequence of a DNA or a mixture of nucleic acids by a peptide assay

摘要

detection of target nucleic acid sequences by pto cleavage and extension assay. The present invention relates to the detection of a target nucleic acid sequence by a ptoc assay (pto cleavage and extension). The present invention detects a target nucleic acid sequence in which the pto (probing and labeling oligonucleotide) is hybridized to the target nucleic acid sequence is cleaved to release a fragment and the fragment is hybridized to the cto (capture and template oligonucleotide). to form an extended duplex, followed by detecting the presence of the extended duplex. Extended duplex provides signals (signal generation, increase, extinction or decrease) from the markings that indicate the presence of extended duplex and has adjustable t-m ~ value, which are well-suited for detecting the presence of nucleic acid sequence target.