Polinucleotu00eddeo that encodes a polypeptide, a nucleic acid molecule, vector, the body not human, polypeptide and method for introducing a nucleic acid of interest in a genome of a human body '

摘要

The present invention is concerned with nuclease fusion proteins and various uses thereof. Specifically, it relates to a polynucleotide encoding a polypeptide comprising (i) a first module comprising at least a first DNA binding domain derived from a homing endonuclease, (ii) a linker, and (iii) a second module comprising at least a second DNA binding domain and a cleavage domain derived from a restriction endonuclease, wherein the polypeptide functionally interacts only with DNA comprising a DNA recognition site for the first DNA binding domain and a DNA recognition site for the second DNA binding domain, and wherein the cleavage domain cleaves DNA within a specific DNA cleavage site upon binding of the polypeptide. Further contemplated are a vector and a non-human transgenic organism comprising the polynucleotide as well as a polypeptide encoded by the polynucleotide of the invention. Finally, the present invention relates to a method for introducing a nucleic acid of interest into a genome of a non-human organism wherein the polypeptide of the invention is applied.