Method for amplification and quantification of light and heavy chain IgG cognate, method of production of monoclonal antibody.Methods of production of Fab fragment of immunoglobulin and immunoglobulin, nucleic acid and kit.

摘要

Herein is reported a method for a multiplex one tube real-time reverse-transcriptase gene-specific polymerase chain reaction for the amplification and quantification of cognate IgG heavy and light chains encoding nucleic acids (human IgG isotype) from a single cell.