proteases capable of hydrolyzing acid ph gluten peptides and proteins from actinomycete actinoallomurus

摘要

Abstract: "Proteases capable of hydrolyzing acidic gluten peptides and proteins of actinomycete actinoallomurus". The present invention relates to a novel family of proteolytic enzymes having the ability of hydrolysis at a pH of between 3 and 8 gluten oligopeptides that are resistant to cleavage by gastric and pancreatic enzymes and whose presence in the intestinal lumen results in toxic effects. The enzymes have been identified as the s8 / s53 endopeptidases and are produced by an actinoallomurus strain. The object of the invention also includes methods for producing the enzyme composition comprising endopeptidases by culturing native, mutant actinoallomurus strains, or recombinant host cells comprising encoding nucleic acids for endopeptidases. said nucleic acids constitute a further object of the invention. enzyme compositions comprising at least one endopeptidase of the invention which are useful for the treatment and / or prevention of celiac psyllosis, dermatitis herpetiformis and any other gluten intolerance disorder as ingredients of pharmaceutical formulations or as food additives and drinks.