method for obtaining 1-questose

摘要

abstract the present invention disclosures an industrial scale method for obtaining 1-kestose by the use of a recombinant fructosyltransferase (ftf), isolated from fescue arundinacea, expressed constitutively in a non-saccharolytic yeast. sucrose: sucrose 1-fructosyltransferase (1-sstrec) is constitutively produced, stable and at high yield, both in the supernatant culture and in intact cells of the host pichia pastoris. hence the invention additionally provides a method for 1-sst production at industrial scale. the recombinant enzyme is then used for mass production of short-chain fructooligosaccharides (fos), specifically 1-kestose, from sucrose. the method of the present invention establishes conditions that allow conversion rates where the synthesized fos were above 55% (w / w) of the total sugars in the reaction mixture and the 1-kestose content reaches values greater than 90% (w / w) of the total fos fraction. Abstract of the Patent Summary: "Method for Obtaining 1-Questose". The present invention discloses a method of obtaining 1-questose, on an industrial scale, using a recombinant fructosyltransferase (ftf), isolated from fescue arundinacea, constitutively expressed in a non-saccharolytic host yeast. In the invention, sucrose: sucrose 1-fructosyltransferase (1-sstrec) recombinant ftf is produced constitutively, stable and at high levels in both the supernatant and cell pellet of a pichia pastoris strain culture. Therefore, the invention also provides a method for obtaining 1-sst on an industrial scale. The resulting recombinant enzyme is then employed for the enzymatic mass production of fructooligosaccharides (fos), specifically 1-questose, from sucrose. The method of the invention allows conversions to fos greater than 55%, with 1-questose being represented by more than 90%.