Optimization of methods of obtaining forming surface and methods of computing surface tension in drop biological samples

摘要

For detecting of surface tension on a medium's border and superficial energies of cells, which participate in immune reactions, usually static methods are used. Five methods are especially widespread. The method of the direct separation of the ring from the surface liquid and method of the maximal pressure of a bubble adapt for research of surface tension on the air-liquid border. The trailing drop method and the rotating drop method are used for gauging a surface indentation on a liquid-liquid border and the method of a motionless drop (method of extremity angles measurement) adopted for detecting the free energy of the surface between liquid and solid. The last method is especially convenient for experimental measurements of interface tension immediately during immunological reactions, but it is seldom used because it is labour-consuming, and there are no sufficiently simple and exact methods for computing surface indentation of a motionless drop. The measurement of geometrical parameters (superficial energy) and the measurement of photometric properties of a drop blood sample during englobement reaction in it were taken as a basis for designing a device for cytophagous activity measurement, where the above parameters are estimated by the changing of intensity of a light passing through the drop sample during the immune reaction.