method for assaying peptidoglycans (pgns) in a glucose polymer sample

摘要

Abstract Method for Assaying Peptidoglycans (pgns) in a Glucose Polymer Sample The present invention relates to a biological method for testing peptidoglycans (pgns) in a sample, particularly a sample of glucose polymers. The pgn assay includes: a) treating the glucose polymer sample by sonication, heating, and / or alkalinization; b) contacting the treated sample or diluting it with a recombinant cell expressing an exogenous tlr2 (toll-like receptor 2) and a reporter gene directly dependent on the signaling pathway associated with tlr2. the reporter gene codes for a colored or fluorescent protein or for a protein the activity of which is measurable with or without a substrate; c) measuring the reporter gene signal; and d) determining the amount of pgn in the sample using a standard curve of correlation between the amount of pgn and the reporter gene signal strength. 1/1