Novel Zinc Oxide Nanorod and Chitosan-Based Electrochemical Glucose Biosensors for Glucose Assay in Human Serum Samples

摘要

In this study, it is presented two glucose biosensors. The first biosensor called Biosensor G was constructed by immobilizing glucose oxidase enzyme on our prepared surface-dependent zinc oxide nanorod prepared. The other called Biosensor GK was fabricated by immobilizing glucose oxidase enzyme on zinc oxide nanorod and chitosan. The optimum operating conditions of two biosensors such as buffer concentration, buffer pH and ambient temperature effect on the biosensors' responses were studied, the shelf life reproducibility, detection range of each enzyme electrodes were also determined. K_m values of biosensor G and GK are found as 0.88 mM and 0.84 mM, respectively. It was determined the maximum speed of biosensor G and biosensor GK as 136.9 mV/min. and 135.1 mV/min., respectively. Additionally, glucose assay in blood serum samples was also successfully carried out by using the standard addition method with Biosensor GK. The results were good agreement with previously reported values.