hla dna typing method, and primer set and hla gene dna typing kit

摘要

1/1 summary “hla dna typing method and primer set and hla gene dna typing kit” purpose is to provide a hla dna typing method and kit. high precision that makes full use of a high throughput sequencer and eliminates ambiguity derived from phase ambiguity. The invention provides a DNA HLA typing method which comprises: (1) a step for providing a primer set which hybridizes each specifically to an upstream region and a region to one. downstream of at least two genes selected from genes belonging to hla class i and hla class ii in a nucleotide sequence of the human genome and amplify under identical conditions; (2) a step for amplifying these at least two genes in a test sample (dna) simultaneously in the same container under identical pct conditions using this primer set; (3) a step for determining the nucleotide sequence of the pct product; and (4) an optional step for conducting a database homology search.