A set for detecting single nucleotide polymorphism in the promoter of myogeneic transcription factor gene MYF5 in cattle

摘要

The present invention relates to a kit for detecting a single nucleotide polymorphism in the promoter of the myogenic transcription factor gene MYF5 in cattle by the SNaPshot multiplexing mini-sequencing method, characterized in that it contains extension primers comprising the composition MYF5pr1_F: 5´ CAT TCT CCT ACC CCT GAT TT 3´, MYF5pr2_R: 5 T CAC TTA GAG GGT GTC ACA GAC 3´, for amplification of the whole MYF5 gene promoter as a specific 1302 bp DNA segment and further includes new SBE extension primers SBEMyf5Pr102F: 5´ (AT) .sub.6.n.GCA GTC AGG GRG GAT CTT CC 3´ for detection of new genetic polymorphism at 102 bp G / A, SBEMyf5Pr145R: 5´ (AT) .sub.3.n.GCT GAG GGG CCT TCT TTT C 3´ for detection of genetic polymorphism at position 145 bp G / A, SBEMyf5Pr149F: 5´ GCT CAG CAA ACA GGC ACA 3´ for detection of genetic polymorphism at 149 bp position G / T, SBEMyf5Pr208R: 5´ (AT) .sub.9.n.TCT CTC TGC TCA TCT GAA GGG 3 'for detection of genetic polymorphism at 208 bp G / A, SBEMyf5 Pr248R: 5´ (AT) .sub.17.n.AGA CTT GAA TAT GAA GTT TTG GAC 3´ for detection of genetic polymorphism at 248 bp G / A, SBEMyf5Pr 290F: 5´ (AT) .sub.11. n.CCA GGT TAC CCC CAA TAA CAT A 3´ for detection of genetic polymorphism at 290 bp position G / C, SBEMyf5Pr529R: 5´ (AT) .sub.15.n.TCG TGT AAA ATT TCC AAC TCC TTC 3´ for detection genetic polymorphism at position 529 bp T / C, SBEMyf5Pr586R: 5 '(AT) .sub.13.n.CAAACTAACACCGTTACAGATGC 3' for detection of genetic polymorphism at 586 bp T / C.