Transformed Escherichia coli cell and method for the assessment of efficiency of washing preparations or techniques in washing of surfaces

摘要

in the disclosed monitoring process cleaning solid surfaces, inter alia. inside the dishwasher, dishes, floors and / or exchange of the existing polluting mikroorganizu00f3w species bacteria, especially present in probiotykach.the declaration also includes evaluation of the effectiveness of washing or scrubbing techniques in surface, which is characterized by the fact that contacts the surface of the bacteria zmywanu0105 indicator, performs a cleaning surface by the test prepara zmywanej here or scrubbing techniques, and then verifies the presence of bacteria biofluorescencyjnu0105 tracer method.with the presence of bacteria on the surface zmywanej ratio indicates insufficient effectiveness test preparation or scrubbing techniques, whereby the indicator is used as the bacterium escherichia coli, preferably escherichia coli strain bl21 (de3), transformowan and the vector ekspresyjnym with nukleotydowu0105 sequence.