COVALENT CONJUGATES OF NUCLEIC ACIDS WITH 1,4-BIS-PHENYLAZO-BENZENE AND PHENYLAZO-PHENAZINE DERIVATIVES AS DARK QUENCHERS OF DONOR ACCEPTOR ENERGY TRANSFER FOR USE E.G. IN AUTOMATED NUCLEIC ACID SYNTHESIS

摘要

The present invention provides a family of dark quenchers, termed Black Hole Quenchers ("BHQs"), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.